Non-treated Ramos and Staurosporine treated Ramos cells were subjected to SDS PAGE followed by western blot with 28953-1-AP (Phospho-MST1 (Thr183)/MST2 (Thr180) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Non-treated Ramos and Jurkat and Staurosporine treated Ramos and Jurkat cells were subjected to SDS PAGE followed by western blot with 80093-1-RR (Phospho-MST1 (Thr183)/MST2 (Thr180) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.25 ug Anti-Human Phospho-MST1 (Thr183)/MST2 (Thr180) (80093-1-RR, Clone:1P6) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Rabbit IgG (KFA002), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80093-1-RR (Phospho-MST1 (Thr183)/MST2 (Thr180) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug CoraLite®488 Anti-Human Phospho-MST1 (Thr183)/MST2 (Thr180) (CL488-80093, Clone:1P6), or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.25 ug CoraLite®647 Anti-Human Phospho-MST1 (Thr183)/MST2 (Thr180) (CL647-80093, Clone:1P6), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Non-treated Ramos and Jurkat and Staurosporine treated Ramos and Jurkat cells were subjected to SDS PAGE followed by western blot with 80093-1-RR (Phospho-MST1 (Thr183)/MST2 (Thr180) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80093-1-PBS in a different storage buffer formulation.

1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.25 ug Anti-Human Phospho-MST1 (Thr183)/MST2 (Thr180) (80093-1-RR, Clone:1P6) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Rabbit IgG (KFA002), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80093-1-PBS in a different storage buffer formulation.

Non-treated HeLa and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80093-1-RR (Phospho-MST1 (Thr183)/MST2 (Thr180) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80093-1-PBS in a different storage buffer formulation.

WB result of MST1 antibody (22245-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MST1 transfected HepG2 cells.

Various lysates were subjected to SDS PAGE followed by western blot with 22245-1-AP (MST1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22245-1-AP (MST1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22245-1-AP (MST1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of HepG2 cells using 22245-1-AP (MST1 antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-rabbit IgG (red).

IP result of anti-MST1 (IP:22245-1-AP, 4ug; Detection:22245-1-AP 1:1000) with HeLa cells lysate 2000ug.

WB result of MST1 antibody (66663-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MST1 transfected HepG2 cells.

Various lysates were subjected to SDS PAGE followed by western blot with 66663-1-Ig (MST1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66663-1-Ig (MST1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66663-1-Ig (MST1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using MST1 antibody (66663-1-Ig, Clone: 2G11C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using KHC1699 (STK4 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using KHC1699 (STK4 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using KHC1699 (STK4 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using KHC1699 (STK4 IHC Kit).